Construct: shRNA TRCN0000298134
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_021280.3-2502s21c1
- DNA Barcode:
- CCAACTTTCAAGTGTGCAGTA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Plcg1 (18803)
- Gene Description:
- phospholipase C, gamma 1
- Transcript:
- RefSeq NM_021280.3 (CURRENT)
- Match location:
- Position 2502 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000298134)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CCAACTTTCAAGTGTGCAGTA
- Hairpin Sequence:
-
5'-CCGG-CCAACTTTCAAGTGTGCAGTA-CTCGAG-TACTGCACACTTGAAAGTTGG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCAACTTTCAAGTGTGCAGTACTCGAGTACTGCACACTTGAAAGTTGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCAACTTTCAAGTGTGCAGTACTCGAGTACTGCACACTTGAAAGTTGG-3'
Other clones with same target sequence:
-
TRCN0000024976
