Construct: shRNA TRCN0000298409

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011523.2-3534s21c1
DNA Barcode:
CCAAGGCTAGAACTGGAATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Synj2 (20975)
Gene Description:
synaptojanin 2
Transcript:
RefSeq NM_011523.2 (CURRENT)
Match location:
Position 3534 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000298409)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAGGCTAGAACTGGAATAA
Hairpin Sequence:
5'-CCGG-CCAAGGCTAGAACTGGAATAA-CTCGAG-TTATTCCAGTTCTAGCCTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAGGCTAGAACTGGAATAACTCGAGTTATTCCAGTTCTAGCCTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAGGCTAGAACTGGAATAACTCGAGTTATTCCAGTTCTAGCCTTGG-3'

Other clones with same target sequence:

  1. TRCN0000080617