Construct: shRNA TRCN0000298462

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001428.2-730s21c1
DNA Barcode:
GAATGTCATCAAGGAGAAATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ENO1 (2023)
Gene Description:
enolase 1
Transcript:
RefSeq NM_001428.2 (NON-CURRENT)
Match location:
Position 730 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000298462)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAATGTCATCAAGGAGAAATA
Hairpin Sequence:
5'-CCGG-GAATGTCATCAAGGAGAAATA-CTCGAG-TATTTCTCCTTGATGACATTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAATGTCATCAAGGAGAAATACTCGAGTATTTCTCCTTGATGACATTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAATGTCATCAAGGAGAAATACTCGAGTATTTCTCCTTGATGACATTC-3'

Other clones with same target sequence:

  1. TRCN0000029326