Construct: shRNA TRCN0000298497

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018081.2-867s21c1
DNA Barcode:
TGATAACATCTTGCGAATTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
WRAP53 (55135)
Gene Description:
WD repeat containing antisense to TP53
Transcript:
RefSeq NM_018081.2 (CURRENT)
Match location:
Position 867 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000298497)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGATAACATCTTGCGAATTTA
Hairpin Sequence:
5'-CCGG-TGATAACATCTTGCGAATTTA-CTCGAG-TAAATTCGCAAGATGTTATCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGATAACATCTTGCGAATTTACTCGAGTAAATTCGCAAGATGTTATCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGATAACATCTTGCGAATTTACTCGAGTAAATTCGCAAGATGTTATCA-3'

Other clones with same target sequence:

(none)