Construct: shRNA TRCN0000298595

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022769.3-1042s21c1
DNA Barcode:
TATGACCCACCTGGGTATAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CRTC3 (64784)
Gene Description:
CREB regulated transcription coactivator 3
Transcript:
RefSeq NM_022769.3 (NON-CURRENT)
Match location:
Position 1042 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000298595)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TATGACCCACCTGGGTATAAG
Hairpin Sequence:
5'-CCGG-TATGACCCACCTGGGTATAAG-CTCGAG-CTTATACCCAGGTGGGTCATA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTATGACCCACCTGGGTATAAGCTCGAGCTTATACCCAGGTGGGTCATATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATATGACCCACCTGGGTATAAGCTCGAGCTTATACCCAGGTGGGTCATA-3'

Other clones with same target sequence:

(none)