Construct: shRNA TRCN0000298647

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006987.2-1586s21c1
DNA Barcode:
GGCTATCTCCGTTGCTATATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RPH3AL (9501)
Gene Description:
rabphilin 3A like (without C2 domains)
Transcript:
RefSeq NM_006987.2 (NON-CURRENT)
Match location:
Position 1586 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000298647)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGCTATCTCCGTTGCTATATT
Hairpin Sequence:
5'-CCGG-GGCTATCTCCGTTGCTATATT-CTCGAG-AATATAGCAACGGAGATAGCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGCTATCTCCGTTGCTATATTCTCGAGAATATAGCAACGGAGATAGCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGCTATCTCCGTTGCTATATTCTCGAGAATATAGCAACGGAGATAGCC-3'

Other clones with same target sequence:

(none)