Construct: shRNA TRCN0000298815

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006437.3-1123s21c1
DNA Barcode:
CCTGGGACTATTGGCTAAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PARP4 (143)
Gene Description:
poly(ADP-ribose) polymerase family member 4
Transcript:
RefSeq NM_006437.3 (NON-CURRENT)
Match location:
Position 1123 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000298815)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGGGACTATTGGCTAAGAA
Hairpin Sequence:
5'-CCGG-CCTGGGACTATTGGCTAAGAA-CTCGAG-TTCTTAGCCAATAGTCCCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGGGACTATTGGCTAAGAACTCGAGTTCTTAGCCAATAGTCCCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGGGACTATTGGCTAAGAACTCGAGTTCTTAGCCAATAGTCCCAGG-3'

Other clones with same target sequence:

  1. TRCN0000052924