Construct: shRNA TRCN0000298963

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006312.3-7979s21c1
DNA Barcode:
GCCTGTCTAAAGCCTTAACTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NCOR2 (9612)
Gene Description:
nuclear receptor corepressor 2
Transcript:
RefSeq NM_006312.3 (NON-CURRENT)
Match location:
Position 7979 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000298963)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTGTCTAAAGCCTTAACTA
Hairpin Sequence:
5'-CCGG-GCCTGTCTAAAGCCTTAACTA-CTCGAG-TAGTTAAGGCTTTAGACAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTGTCTAAAGCCTTAACTACTCGAGTAGTTAAGGCTTTAGACAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTGTCTAAAGCCTTAACTACTCGAGTAGTTAAGGCTTTAGACAGGC-3'

Other clones with same target sequence:

  1. TRCN0000060703