Construct: shRNA TRCN0000299025

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005964.1-6115s21c1
DNA Barcode:
CGGGATTCCTTCCTGAAAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MYH10 (4628)
Gene Description:
myosin heavy chain 10
Transcript:
RefSeq NM_005964.1 (NON-CURRENT)
Match location:
Position 6115 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000299025)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGGATTCCTTCCTGAAAGAT
Hairpin Sequence:
5'-CCGG-CGGGATTCCTTCCTGAAAGAT-CTCGAG-ATCTTTCAGGAAGGAATCCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGGATTCCTTCCTGAAAGATCTCGAGATCTTTCAGGAAGGAATCCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGGATTCCTTCCTGAAAGATCTCGAGATCTTTCAGGAAGGAATCCCG-3'

Other clones with same target sequence:

  1. TRCN0000123074