Construct: shRNA TRCN0000299055

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003100.2-619s21c1
DNA Barcode:
GCACAGCAAATTAGCAAGCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SNX2 (6643)
Gene Description:
sorting nexin 2
Transcript:
RefSeq NM_003100.2 (NON-CURRENT)
Match location:
Position 619 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000299055)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACAGCAAATTAGCAAGCAA
Hairpin Sequence:
5'-CCGG-GCACAGCAAATTAGCAAGCAA-CTCGAG-TTGCTTGCTAATTTGCTGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACAGCAAATTAGCAAGCAACTCGAGTTGCTTGCTAATTTGCTGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACAGCAAATTAGCAAGCAACTCGAGTTGCTTGCTAATTTGCTGTGC-3'

Other clones with same target sequence:

  1. TRCN0000064994