Construct: shRNA TRCN0000299153
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_181873.3-1450s21c1
- DNA Barcode:
- GCGGACAGTTAAACTCCTATA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MTMR11 (10903)
- Gene Description:
- myotubularin related protein 11
- Transcript:
- RefSeq NM_181873.3 (CURRENT)
- Match location:
- Position 1450 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000299153)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCGGACAGTTAAACTCCTATA
- Hairpin Sequence:
-
5'-CCGG-GCGGACAGTTAAACTCCTATA-CTCGAG-TATAGGAGTTTAACTGTCCGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCGGACAGTTAAACTCCTATACTCGAGTATAGGAGTTTAACTGTCCGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCGGACAGTTAAACTCCTATACTCGAGTATAGGAGTTTAACTGTCCGC-3'
Other clones with same target sequence:
-
TRCN0000113769
