Construct: shRNA TRCN0000299170

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006109.3-235s21c1
DNA Barcode:
GCGTTTCAAGAGGGAGTTCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRMT5 (10419)
Gene Description:
protein arginine methyltransferase 5
Transcript:
RefSeq NM_006109.3 (NON-CURRENT)
Match location:
Position 235 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000299170)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGTTTCAAGAGGGAGTTCAT
Hairpin Sequence:
5'-CCGG-GCGTTTCAAGAGGGAGTTCAT-CTCGAG-ATGAACTCCCTCTTGAAACGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGTTTCAAGAGGGAGTTCATCTCGAGATGAACTCCCTCTTGAAACGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGTTTCAAGAGGGAGTTCATCTCGAGATGAACTCCCTCTTGAAACGC-3'

Other clones with same target sequence:

  1. TRCN0000292682
  2. TRCN0000107088
  3. TRCN0000182693