Construct: shRNA TRCN0000299203

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_181873.3-1554s21c1
DNA Barcode:
CGTTATAGCAATGCACAGATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MTMR11 (10903)
Gene Description:
myotubularin related protein 11
Transcript:
RefSeq NM_181873.3 (CURRENT)
Match location:
Position 1554 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000299203)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTTATAGCAATGCACAGATA
Hairpin Sequence:
5'-CCGG-CGTTATAGCAATGCACAGATA-CTCGAG-TATCTGTGCATTGCTATAACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTTATAGCAATGCACAGATACTCGAGTATCTGTGCATTGCTATAACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTTATAGCAATGCACAGATACTCGAGTATCTGTGCATTGCTATAACG-3'

Other clones with same target sequence:

  1. TRCN0000113770