Construct: shRNA TRCN0000299209

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003127.2-1347s21c1
DNA Barcode:
GCCCATGAAGACAGCTTCAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SPTAN1 (6709)
Gene Description:
spectrin alpha, non-erythrocytic 1
Transcript:
RefSeq NM_003127.2 (NON-CURRENT)
Match location:
Position 1347 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000299209)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCATGAAGACAGCTTCAAA
Hairpin Sequence:
5'-CCGG-GCCCATGAAGACAGCTTCAAA-CTCGAG-TTTGAAGCTGTCTTCATGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCATGAAGACAGCTTCAAACTCGAGTTTGAAGCTGTCTTCATGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCATGAAGACAGCTTCAAACTCGAGTTTGAAGCTGTCTTCATGGGC-3'

Other clones with same target sequence:

  1. TRCN0000053668