Construct: shRNA TRCN0000299229

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015439.2-791s21c1
DNA Barcode:
GCAGGAGAAATTAGCTCGCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CCDC28A (25901)
Gene Description:
coiled-coil domain containing 28A
Transcript:
RefSeq NM_015439.2 (CURRENT)
Match location:
Position 791 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000299229)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGGAGAAATTAGCTCGCTT
Hairpin Sequence:
5'-CCGG-GCAGGAGAAATTAGCTCGCTT-CTCGAG-AAGCGAGCTAATTTCTCCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGGAGAAATTAGCTCGCTTCTCGAGAAGCGAGCTAATTTCTCCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGGAGAAATTAGCTCGCTTCTCGAGAAGCGAGCTAATTTCTCCTGC-3'

Other clones with same target sequence:

  1. TRCN0000063111