Construct: shRNA TRCN0000299255

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014713.4-991s21c1
DNA Barcode:
CCTGTTCATTGTTCTTGTGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LAPTM4A (9741)
Gene Description:
lysosomal protein transmembrane 4 alpha
Transcript:
RefSeq NM_014713.4 (NON-CURRENT)
Match location:
Position 991 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000299255)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGTTCATTGTTCTTGTGTT
Hairpin Sequence:
5'-CCGG-CCTGTTCATTGTTCTTGTGTT-CTCGAG-AACACAAGAACAATGAACAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGTTCATTGTTCTTGTGTTCTCGAGAACACAAGAACAATGAACAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGTTCATTGTTCTTGTGTTCTCGAGAACACAAGAACAATGAACAGG-3'

Other clones with same target sequence:

  1. TRCN0000059984