Construct: shRNA TRCN0000299268

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003255.4-866s21c1
DNA Barcode:
CAAGTTCTTCGCCTGCATCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TIMP2 (7077)
Gene Description:
TIMP metallopeptidase inhibitor 2
Transcript:
RefSeq NM_003255.4 (NON-CURRENT)
Match location:
Position 866 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000299268)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAGTTCTTCGCCTGCATCAA
Hairpin Sequence:
5'-CCGG-CAAGTTCTTCGCCTGCATCAA-CTCGAG-TTGATGCAGGCGAAGAACTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAGTTCTTCGCCTGCATCAACTCGAGTTGATGCAGGCGAAGAACTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAGTTCTTCGCCTGCATCAACTCGAGTTGATGCAGGCGAAGAACTTG-3'

Other clones with same target sequence:

  1. TRCN0000052437