Construct: shRNA TRCN0000299287

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012250.4-647s21c1
DNA Barcode:
CTCAGAGTAAAGGATCGTGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RRAS2 (22800)
Gene Description:
RAS related 2
Transcript:
RefSeq NM_012250.4 (NON-CURRENT)
Match location:
Position 647 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000299287)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCAGAGTAAAGGATCGTGAT
Hairpin Sequence:
5'-CCGG-CTCAGAGTAAAGGATCGTGAT-CTCGAG-ATCACGATCCTTTACTCTGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCAGAGTAAAGGATCGTGATCTCGAGATCACGATCCTTTACTCTGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCAGAGTAAAGGATCGTGATCTCGAGATCACGATCCTTTACTCTGAG-3'

Other clones with same target sequence:

  1. TRCN0000047727