Construct: shRNA TRCN0000299397

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019063.3-2544s21c1
DNA Barcode:
CGGATTGTAAGGACATTGATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EML4 (27436)
Gene Description:
EMAP like 4
Transcript:
RefSeq NM_019063.3 (NON-CURRENT)
Match location:
Position 2544 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000299397)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGATTGTAAGGACATTGATT
Hairpin Sequence:
5'-CCGG-CGGATTGTAAGGACATTGATT-CTCGAG-AATCAATGTCCTTACAATCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGATTGTAAGGACATTGATTCTCGAGAATCAATGTCCTTACAATCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGATTGTAAGGACATTGATTCTCGAGAATCAATGTCCTTACAATCCG-3'

Other clones with same target sequence:

  1. TRCN0000117341