Construct: shRNA TRCN0000299424

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006463.3-1301s21c1
DNA Barcode:
TCCAGGAAACTGGATTCTTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STAMBP (10617)
Gene Description:
STAM binding protein
Transcript:
RefSeq NM_006463.3 (NON-CURRENT)
Match location:
Position 1301 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000299424)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCAGGAAACTGGATTCTTTA
Hairpin Sequence:
5'-CCGG-TCCAGGAAACTGGATTCTTTA-CTCGAG-TAAAGAATCCAGTTTCCTGGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCAGGAAACTGGATTCTTTACTCGAGTAAAGAATCCAGTTTCCTGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCAGGAAACTGGATTCTTTACTCGAGTAAAGAATCCAGTTTCCTGGA-3'

Other clones with same target sequence:

  1. TRCN0000073976