Construct: shRNA TRCN0000299480

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000224.2-748s21c1
DNA Barcode:
CTTCATGAAGAAGAACCACGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KRT18 (3875)
Gene Description:
keratin 18
Transcript:
RefSeq NM_000224.2 (NON-CURRENT)
Match location:
Position 748 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000299480)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTCATGAAGAAGAACCACGA
Hairpin Sequence:
5'-CCGG-CTTCATGAAGAAGAACCACGA-CTCGAG-TCGTGGTTCTTCTTCATGAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTCATGAAGAAGAACCACGACTCGAGTCGTGGTTCTTCTTCATGAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTCATGAAGAAGAACCACGACTCGAGTCGTGGTTCTTCTTCATGAAG-3'

Other clones with same target sequence:

  1. TRCN0000062366