Construct: shRNA TRCN0000299568

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000814.4-497s21c1
DNA Barcode:
CCCGACACATATTTCTTAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GABRB3 (2562)
Gene Description:
gamma-aminobutyric acid type A receptor beta3 subunit
Transcript:
RefSeq NM_000814.4 (NON-CURRENT)
Match location:
Position 497 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000299568)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCGACACATATTTCTTAAAT
Hairpin Sequence:
5'-CCGG-CCCGACACATATTTCTTAAAT-CTCGAG-ATTTAAGAAATATGTGTCGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCGACACATATTTCTTAAATCTCGAGATTTAAGAAATATGTGTCGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCGACACATATTTCTTAAATCTCGAGATTTAAGAAATATGTGTCGGG-3'

Other clones with same target sequence:

  1. TRCN0000061490