Construct: shRNA TRCN0000299575

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003224.3-876s21c1
DNA Barcode:
CGAAGACAAACTTTCCTCTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ARFRP1 (10139)
Gene Description:
ADP ribosylation factor related protein 1
Transcript:
RefSeq NM_003224.3 (NON-CURRENT)
Match location:
Position 876 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000299575)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAAGACAAACTTTCCTCTAT
Hairpin Sequence:
5'-CCGG-CGAAGACAAACTTTCCTCTAT-CTCGAG-ATAGAGGAAAGTTTGTCTTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAAGACAAACTTTCCTCTATCTCGAGATAGAGGAAAGTTTGTCTTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAAGACAAACTTTCCTCTATCTCGAGATAGAGGAAAGTTTGTCTTCG-3'

Other clones with same target sequence:

  1. TRCN0000047758