Construct: shRNA TRCN0000299576

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001968.3-1566s21c1
DNA Barcode:
CCGACTACAGAAGAGGAGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EIF4E (1977)
Gene Description:
eukaryotic translation initiation factor 4E
Transcript:
RefSeq NM_001968.3 (NON-CURRENT)
Match location:
Position 1566 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000299576)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGACTACAGAAGAGGAGAAA
Hairpin Sequence:
5'-CCGG-CCGACTACAGAAGAGGAGAAA-CTCGAG-TTTCTCCTCTTCTGTAGTCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGACTACAGAAGAGGAGAAACTCGAGTTTCTCCTCTTCTGTAGTCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGACTACAGAAGAGGAGAAACTCGAGTTTCTCCTCTTCTGTAGTCGG-3'

Other clones with same target sequence:

  1. TRCN0000062575