Construct: shRNA TRCN0000299585

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001191.2-857s21c1
DNA Barcode:
GCTCACTCTTCAGTCGGAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BCL2L1 (598)
Gene Description:
BCL2 like 1
Transcript:
RefSeq NM_001191.2 (NON-CURRENT)
Match location:
Position 857 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000299585)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCACTCTTCAGTCGGAAAT
Hairpin Sequence:
5'-CCGG-GCTCACTCTTCAGTCGGAAAT-CTCGAG-ATTTCCGACTGAAGAGTGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCACTCTTCAGTCGGAAATCTCGAGATTTCCGACTGAAGAGTGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCACTCTTCAGTCGGAAATCTCGAGATTTCCGACTGAAGAGTGAGC-3'

Other clones with same target sequence:

  1. TRCN0000033499