Construct: shRNA TRCN0000299734

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005711.3-859s21c1
DNA Barcode:
GCGAATTTATGGGAAGAAATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EDIL3 (10085)
Gene Description:
EGF like repeats and discoidin domains 3
Transcript:
RefSeq NM_005711.3 (NON-CURRENT)
Match location:
Position 859 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000299734)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGAATTTATGGGAAGAAATT
Hairpin Sequence:
5'-CCGG-GCGAATTTATGGGAAGAAATT-CTCGAG-AATTTCTTCCCATAAATTCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGAATTTATGGGAAGAAATTCTCGAGAATTTCTTCCCATAAATTCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGAATTTATGGGAAGAAATTCTCGAGAATTTCTTCCCATAAATTCGC-3'

Other clones with same target sequence:

  1. TRCN0000053424