Construct: shRNA TRCN0000299768
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_016141.2-560s21c1
- DNA Barcode:
- GCTTTGGATTCTTTACAGAAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- DYNC1LI1 (51143)
- Gene Description:
- dynein cytoplasmic 1 light intermediate chain 1
- Transcript:
- RefSeq NM_016141.2 (NON-CURRENT)
- Match location:
- Position 560 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000299768)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCTTTGGATTCTTTACAGAAA
- Hairpin Sequence:
-
5'-CCGG-GCTTTGGATTCTTTACAGAAA-CTCGAG-TTTCTGTAAAGAATCCAAAGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCTTTGGATTCTTTACAGAAACTCGAGTTTCTGTAAAGAATCCAAAGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCTTTGGATTCTTTACAGAAACTCGAGTTTCTGTAAAGAATCCAAAGC-3'
Other clones with same target sequence:
-
TRCN0000072980
