Construct: shRNA TRCN0000299787

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004282.3-846s21c1
DNA Barcode:
GATCAGAAGTTTCAATCCATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BAG2 (9532)
Gene Description:
BAG cochaperone 2
Transcript:
RefSeq NM_004282.3 (NON-CURRENT)
Match location:
Position 846 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000299787)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATCAGAAGTTTCAATCCATA
Hairpin Sequence:
5'-CCGG-GATCAGAAGTTTCAATCCATA-CTCGAG-TATGGATTGAAACTTCTGATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATCAGAAGTTTCAATCCATACTCGAGTATGGATTGAAACTTCTGATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATCAGAAGTTTCAATCCATACTCGAGTATGGATTGAAACTTCTGATC-3'

Other clones with same target sequence:

  1. TRCN0000033591