Construct: shRNA TRCN0000299894

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006330.2-727s21c1
DNA Barcode:
CCCTGATGTTTGGTTCTCTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LYPLA1 (10434)
Gene Description:
lysophospholipase 1
Transcript:
RefSeq NM_006330.2 (NON-CURRENT)
Match location:
Position 727 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000299894)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTGATGTTTGGTTCTCTTA
Hairpin Sequence:
5'-CCGG-CCCTGATGTTTGGTTCTCTTA-CTCGAG-TAAGAGAACCAAACATCAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTGATGTTTGGTTCTCTTACTCGAGTAAGAGAACCAAACATCAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTGATGTTTGGTTCTCTTACTCGAGTAAGAGAACCAAACATCAGGG-3'

Other clones with same target sequence:

  1. TRCN0000052064