Construct: shRNA TRCN0000300037

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006750.3-611s21c1
DNA Barcode:
CCGAGAAGTAACACCATATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SNTB2 (6645)
Gene Description:
syntrophin beta 2
Transcript:
RefSeq NM_006750.3 (CURRENT)
Match location:
Position 611 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000300037)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGAGAAGTAACACCATATAT
Hairpin Sequence:
5'-CCGG-CCGAGAAGTAACACCATATAT-CTCGAG-ATATATGGTGTTACTTCTCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGAGAAGTAACACCATATATCTCGAGATATATGGTGTTACTTCTCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGAGAAGTAACACCATATATCTCGAGATATATGGTGTTACTTCTCGG-3'

Other clones with same target sequence:

  1. TRCN0000053524