Construct: shRNA TRCN0000300123

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022154.5-1623s21c1
DNA Barcode:
CCTTGCTATTCAACTTCCTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC39A8 (64116)
Gene Description:
solute carrier family 39 member 8
Transcript:
RefSeq NM_022154.5 (CURRENT)
Match location:
Position 1623 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000300123)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTGCTATTCAACTTCCTTT
Hairpin Sequence:
5'-CCGG-CCTTGCTATTCAACTTCCTTT-CTCGAG-AAAGGAAGTTGAATAGCAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTGCTATTCAACTTCCTTTCTCGAGAAAGGAAGTTGAATAGCAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTGCTATTCAACTTCCTTTCTCGAGAAAGGAAGTTGAATAGCAAGG-3'

Other clones with same target sequence:

  1. TRCN0000043690