Construct: shRNA TRCN0000300184

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012434.4-1005s21c1
DNA Barcode:
GCTATCGTAGTTGCACACTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC17A5 (26503)
Gene Description:
solute carrier family 17 member 5
Transcript:
RefSeq NM_012434.4 (NON-CURRENT)
Match location:
Position 1005 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000300184)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTATCGTAGTTGCACACTTT
Hairpin Sequence:
5'-CCGG-GCTATCGTAGTTGCACACTTT-CTCGAG-AAAGTGTGCAACTACGATAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTATCGTAGTTGCACACTTTCTCGAGAAAGTGTGCAACTACGATAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTATCGTAGTTGCACACTTTCTCGAGAAAGTGTGCAACTACGATAGC-3'

Other clones with same target sequence:

  1. TRCN0000043625