Construct: shRNA TRCN0000300244

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005312.2-2896s21c1
DNA Barcode:
GCTGAATAACTTCAACTCCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RAPGEF1 (2889)
Gene Description:
Rap guanine nucleotide exchange factor 1
Transcript:
RefSeq NM_005312.2 (NON-CURRENT)
Match location:
Position 2896 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000300244)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGAATAACTTCAACTCCTA
Hairpin Sequence:
5'-CCGG-GCTGAATAACTTCAACTCCTA-CTCGAG-TAGGAGTTGAAGTTATTCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGAATAACTTCAACTCCTACTCGAGTAGGAGTTGAAGTTATTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGAATAACTTCAACTCCTACTCGAGTAGGAGTTGAAGTTATTCAGC-3'

Other clones with same target sequence:

  1. TRCN0000048131