Construct: shRNA TRCN0000300275

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003838.2-1536s21c1
DNA Barcode:
GCTTAGATGTTTGGAATCTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FPGT (8790)
Gene Description:
fucose-1-phosphate guanylyltransferase
Transcript:
RefSeq NM_003838.2 (NON-CURRENT)
Match location:
Position 1536 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000300275)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTAGATGTTTGGAATCTTA
Hairpin Sequence:
5'-CCGG-GCTTAGATGTTTGGAATCTTA-CTCGAG-TAAGATTCCAAACATCTAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTAGATGTTTGGAATCTTACTCGAGTAAGATTCCAAACATCTAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTAGATGTTTGGAATCTTACTCGAGTAAGATTCCAAACATCTAAGC-3'

Other clones with same target sequence:

  1. TRCN0000035805