Construct: shRNA TRCN0000300284

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175610.2-2743s21c1
DNA Barcode:
GCGATCTCATAAACTTCGTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TJP1 (7082)
Gene Description:
tight junction protein 1
Transcript:
RefSeq NM_175610.2 (NON-CURRENT)
Match location:
Position 2743 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000300284)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGATCTCATAAACTTCGTAA
Hairpin Sequence:
5'-CCGG-GCGATCTCATAAACTTCGTAA-CTCGAG-TTACGAAGTTTATGAGATCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGATCTCATAAACTTCGTAACTCGAGTTACGAAGTTTATGAGATCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGATCTCATAAACTTCGTAACTCGAGTTACGAAGTTTATGAGATCGC-3'

Other clones with same target sequence:

  1. TRCN0000116253