Construct: shRNA TRCN0000300289

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004434.2-3279s21c1
DNA Barcode:
GCCACCTTCTTGTGTATATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EML1 (2009)
Gene Description:
EMAP like 1
Transcript:
RefSeq NM_004434.2 (NON-CURRENT)
Match location:
Position 3279 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000300289)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCACCTTCTTGTGTATATTA
Hairpin Sequence:
5'-CCGG-GCCACCTTCTTGTGTATATTA-CTCGAG-TAATATACACAAGAAGGTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCACCTTCTTGTGTATATTACTCGAGTAATATACACAAGAAGGTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCACCTTCTTGTGTATATTACTCGAGTAATATACACAAGAAGGTGGC-3'

Other clones with same target sequence:

  1. TRCN0000116287