Construct: shRNA TRCN0000300309

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018092.3-671s21c1
DNA Barcode:
CCATCATTTGAGTGTCGGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NETO2 (81831)
Gene Description:
neuropilin and tolloid like 2
Transcript:
RefSeq NM_018092.3 (NON-CURRENT)
Match location:
Position 671 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000300309)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATCATTTGAGTGTCGGTTT
Hairpin Sequence:
5'-CCGG-CCATCATTTGAGTGTCGGTTT-CTCGAG-AAACCGACACTCAAATGATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATCATTTGAGTGTCGGTTTCTCGAGAAACCGACACTCAAATGATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATCATTTGAGTGTCGGTTTCTCGAGAAACCGACACTCAAATGATGG-3'

Other clones with same target sequence:

  1. TRCN0000118473