Construct: shRNA TRCN0000300416
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_001613.2-737s21c1
- DNA Barcode:
- GCGTGAGATTGTCCGGGACAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ACTA2 (59)
- Gene Description:
- actin alpha 2, smooth muscle
- Transcript:
- RefSeq NM_001613.2 (NON-CURRENT)
- Match location:
- Position 737 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000300416)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCGTGAGATTGTCCGGGACAT
- Hairpin Sequence:
-
5'-CCGG-GCGTGAGATTGTCCGGGACAT-CTCGAG-ATGTCCCGGACAATCTCACGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCGTGAGATTGTCCGGGACATCTCGAGATGTCCCGGACAATCTCACGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCGTGAGATTGTCCGGGACATCTCGAGATGTCCCGGACAATCTCACGC-3'
Other clones with same target sequence:
-
TRCN0000116935
