Construct: shRNA TRCN0000300435

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005277.3-1709s21c1
DNA Barcode:
CCCTCAGATTACTCATGTTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GPM6A (2823)
Gene Description:
glycoprotein M6A
Transcript:
RefSeq NM_005277.3 (NON-CURRENT)
Match location:
Position 1709 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000300435)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTCAGATTACTCATGTTTA
Hairpin Sequence:
5'-CCGG-CCCTCAGATTACTCATGTTTA-CTCGAG-TAAACATGAGTAATCTGAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTCAGATTACTCATGTTTACTCGAGTAAACATGAGTAATCTGAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTCAGATTACTCATGTTTACTCGAGTAAACATGAGTAATCTGAGGG-3'

Other clones with same target sequence:

  1. TRCN0000117242