Construct: shRNA TRCN0000300504

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003542.3-73s21c1
DNA Barcode:
GATAACATCCAGGGCATTACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
H4C3 (8364)
Gene Description:
H4 clustered histone 3
Transcript:
RefSeq NM_003542.3 (CURRENT)
Match location:
Position 73 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000300504)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATAACATCCAGGGCATTACA
Hairpin Sequence:
5'-CCGG-GATAACATCCAGGGCATTACA-CTCGAG-TGTAATGCCCTGGATGTTATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATAACATCCAGGGCATTACACTCGAGTGTAATGCCCTGGATGTTATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATAACATCCAGGGCATTACACTCGAGTGTAATGCCCTGGATGTTATC-3'

Other clones with same target sequence:

  1. TRCN0000106959