Construct: shRNA TRCN0000300526

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014774.2-2281s21c1
DNA Barcode:
GCACTGAAGATCTTCAGGATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EFCAB14 (9813)
Gene Description:
EF-hand calcium binding domain 14
Transcript:
RefSeq NM_014774.2 (NON-CURRENT)
Match location:
Position 2281 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000300526)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACTGAAGATCTTCAGGATT
Hairpin Sequence:
5'-CCGG-GCACTGAAGATCTTCAGGATT-CTCGAG-AATCCTGAAGATCTTCAGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACTGAAGATCTTCAGGATTCTCGAGAATCCTGAAGATCTTCAGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACTGAAGATCTTCAGGATTCTCGAGAATCCTGAAGATCTTCAGTGC-3'

Other clones with same target sequence:

  1. TRCN0000053326