Construct: shRNA TRCN0000300534

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004457.3-1282s21c1
DNA Barcode:
GCCCTCAGATATTGCAGTAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ACSL3 (2181)
Gene Description:
acyl-CoA synthetase long chain family member 3
Transcript:
RefSeq NM_004457.3 (NON-CURRENT)
Match location:
Position 1282 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000300534)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCTCAGATATTGCAGTAAT
Hairpin Sequence:
5'-CCGG-GCCCTCAGATATTGCAGTAAT-CTCGAG-ATTACTGCAATATCTGAGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCTCAGATATTGCAGTAATCTCGAGATTACTGCAATATCTGAGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCTCAGATATTGCAGTAATCTCGAGATTACTGCAATATCTGAGGGC-3'

Other clones with same target sequence:

  1. TRCN0000045528