Construct: shRNA TRCN0000300549

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012401.2-5500s21c1
DNA Barcode:
GCAGAAGTACTATGACGAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PLXNB2 (23654)
Gene Description:
plexin B2
Transcript:
RefSeq NM_012401.2 (NON-CURRENT)
Match location:
Position 5500 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000300549)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGAAGTACTATGACGAGAT
Hairpin Sequence:
5'-CCGG-GCAGAAGTACTATGACGAGAT-CTCGAG-ATCTCGTCATAGTACTTCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGAAGTACTATGACGAGATCTCGAGATCTCGTCATAGTACTTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGAAGTACTATGACGAGATCTCGAGATCTCGTCATAGTACTTCTGC-3'

Other clones with same target sequence:

  1. TRCN0000048189