Construct: shRNA TRCN0000300581

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021098.2-5756s21c1
DNA Barcode:
GCCCGTCTACTTCGTGACCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CACNA1H (8912)
Gene Description:
calcium voltage-gated channel subunit alpha1 H
Transcript:
RefSeq NM_021098.2 (NON-CURRENT)
Match location:
Position 5756 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000300581)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCGTCTACTTCGTGACCTT
Hairpin Sequence:
5'-CCGG-GCCCGTCTACTTCGTGACCTT-CTCGAG-AAGGTCACGAAGTAGACGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCGTCTACTTCGTGACCTTCTCGAGAAGGTCACGAAGTAGACGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCGTCTACTTCGTGACCTTCTCGAGAAGGTCACGAAGTAGACGGGC-3'

Other clones with same target sequence:

  1. TRCN0000044212