Construct: shRNA TRCN0000300619

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014584.1-1498s21c1
DNA Barcode:
GCCAGAAAGTGGACCTAGTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ERO1A (30001)
Gene Description:
endoplasmic reticulum oxidoreductase 1 alpha
Transcript:
RefSeq NM_014584.1 (NON-CURRENT)
Match location:
Position 1498 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000300619)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAGAAAGTGGACCTAGTTA
Hairpin Sequence:
5'-CCGG-GCCAGAAAGTGGACCTAGTTA-CTCGAG-TAACTAGGTCCACTTTCTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAGAAAGTGGACCTAGTTACTCGAGTAACTAGGTCCACTTTCTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAGAAAGTGGACCTAGTTACTCGAGTAACTAGGTCCACTTTCTGGC-3'

Other clones with same target sequence:

  1. TRCN0000059755