Construct: shRNA TRCN0000300759

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_057175.3-1819s21c1
DNA Barcode:
CCAGTTTGACTTTCATACATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NAA15 (80155)
Gene Description:
N(alpha)-acetyltransferase 15, NatA auxiliary subunit
Transcript:
RefSeq NM_057175.3 (NON-CURRENT)
Match location:
Position 1819 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000300759)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGTTTGACTTTCATACATA
Hairpin Sequence:
5'-CCGG-CCAGTTTGACTTTCATACATA-CTCGAG-TATGTATGAAAGTCAAACTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGTTTGACTTTCATACATACTCGAGTATGTATGAAAGTCAAACTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGTTTGACTTTCATACATACTCGAGTATGTATGAAAGTCAAACTGG-3'

Other clones with same target sequence:

  1. TRCN0000061332