Construct: shRNA TRCN0000300847

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015533.3-2102s21c1
DNA Barcode:
CCCTCTAAGTTGAGCAGGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TKFC (26007)
Gene Description:
triokinase and FMN cyclase
Transcript:
RefSeq NM_015533.3 (NON-CURRENT)
Match location:
Position 2102 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000300847)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTCTAAGTTGAGCAGGAAA
Hairpin Sequence:
5'-CCGG-CCCTCTAAGTTGAGCAGGAAA-CTCGAG-TTTCCTGCTCAACTTAGAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTCTAAGTTGAGCAGGAAACTCGAGTTTCCTGCTCAACTTAGAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTCTAAGTTGAGCAGGAAACTCGAGTTTCCTGCTCAACTTAGAGGG-3'

Other clones with same target sequence:

  1. TRCN0000078618