Construct: shRNA TRCN0000300851

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001013703.2-334s21c1
DNA Barcode:
CCAAAGGTCTATCAAATGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EIF2AK4 (440275)
Gene Description:
eukaryotic translation initiation factor 2 alpha kinase 4
Transcript:
RefSeq NM_001013703.2 (NON-CURRENT)
Match location:
Position 334 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000300851)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAAGGTCTATCAAATGAAA
Hairpin Sequence:
5'-CCGG-CCAAAGGTCTATCAAATGAAA-CTCGAG-TTTCATTTGATAGACCTTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAAGGTCTATCAAATGAAACTCGAGTTTCATTTGATAGACCTTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAAGGTCTATCAAATGAAACTCGAGTTTCATTTGATAGACCTTTGG-3'

Other clones with same target sequence:

  1. TRCN0000078651