Construct: shRNA TRCN0000300963

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_174929.2-3121s21c1
DNA Barcode:
CCAGCATTGATCCTTCTGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZMIZ2 (83637)
Gene Description:
zinc finger MIZ-type containing 2
Transcript:
RefSeq NM_174929.2 (CURRENT)
Match location:
Position 3121 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000300963)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGCATTGATCCTTCTGTTT
Hairpin Sequence:
5'-CCGG-CCAGCATTGATCCTTCTGTTT-CTCGAG-AAACAGAAGGATCAATGCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGCATTGATCCTTCTGTTTCTCGAGAAACAGAAGGATCAATGCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGCATTGATCCTTCTGTTTCTCGAGAAACAGAAGGATCAATGCTGG-3'

Other clones with same target sequence:

  1. TRCN0000108195