Construct: shRNA TRCN0000300979

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_182926.2-3501s21c1
DNA Barcode:
GCTGTTGAGTTGGAGGCTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KTN1 (3895)
Gene Description:
kinectin 1
Transcript:
RefSeq NM_182926.2 (NON-CURRENT)
Match location:
Position 3501 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000300979)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTTGAGTTGGAGGCTAAA
Hairpin Sequence:
5'-CCGG-GCTGTTGAGTTGGAGGCTAAA-CTCGAG-TTTAGCCTCCAACTCAACAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTTGAGTTGGAGGCTAAACTCGAGTTTAGCCTCCAACTCAACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTTGAGTTGGAGGCTAAACTCGAGTTTAGCCTCCAACTCAACAGC-3'

Other clones with same target sequence:

  1. TRCN0000063520